3′ and 5´ RACE (System for Rapid Amplification of cDNA Ends)


Kinda get the objective but the steps are a bit complicated for me

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So the 3′ and 5′ is a naming convention that relates to organic chemistry, and how we name the carbon positions in atoms. [This image](https://images.app.goo.gl/x3CfLiG4VhjuuSJ18) of ribose and deoxyribose show us the systematic numbering of the carbon ring structure. Note how both the ribose and deoxyribose share the same 3 position and 5 position, which is why the 3′ and 5′ ends are the same for RNA and DNA

Ultimately, when it comes to DNA/RNA, the 5′ end has a phosphate exposed, while the 3′ end has a carbohydrate (hydroxyl) end exposed. This difference explains why it is important to differentiate between the two ends in terms of what chemistry can happen at each location.