De novo peptide sequencing

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I’m working on a graphic design task for a company with scientists who work on cell sciences. They want me to do icons for said post title. They’re great at their job in their field of science, but not as great to explain their work to someone as simple as me (or maybe I got porridge for brains). Could someone put there explain step by step on what this is?

In: Biology

Anonymous 0 Comments

It’s a way of finding out the amino acid sequence of a protein without any prior knowledge about it (hence de novo). The protein gets blasted apart with electricity and laser light and the fragments are analyzed with something called a mass spectrometer. Clever math can then be used on the output of that to determine what amino acids in what order make up the protein.