Your original assumption would be correct. Assuming the drivetrain is rigidly coupled and not slipping, achieving the same speed in the same gear will result in the same engine speed. It’s a fixed relationship between the engine and wheel.
Either there’s something that’s variable (the clutch slipping for example) or your measurements are incorrect. It definitely wouldn’t change by 400 RPM. The tachometer or speedometer could be faulty too.
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