In the specific 1938 experiment they used a mixture of radium and beryllium. Radium emits alpha particles and beryllium emits neutrons when bombarded with alpha particles.
Nowadays they use what is called neutron spallation sources (there are many great explanations of this process on the internet)
They understood Uranium was split as it was the only way to explain that they found a bunch of lighter atoms in the sample that weren’t there before.
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