It depend on the organ and for how long it is being transported. Alive is a relative term though. Organs can generally be disconnected from blood vanes and nerves and therefore die but unless it decomposes it will just work again once it is connected back again. So keeping an organ usable is much about preventing decomposition. So keeping it cold, seal it from possible infectious sources, and protect it from physical damage. Very similar to how you keep food safe to eat.
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