Deer are generally prey animals and their only defence is their ability to run fast. As a herd animal they are sensitive to any dangers that may be in their environment, so slight rustling of dangers or gunshots will send them off. They also may freeze in other threading situations.
But cars are different because they travel so fast which deer don’t expect, the headlights generally dazzle their vision in the middle of the night when their eyes are focused for the dark. Any traffic coming is hard to judge due to the blinding and unfamiliar noise of a car coming at speed.
Basically, they evolved to be extra sensitive to their environment during the day and night but not for large speeding vehicles.
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