Separation of church and state is more of a doctrine that Pres Jefferson wrote in the early 1800s. It is not part of the constitution. The US Constitution forbids the government from establishing religion or to restrict the free exercise of religion.
The statement “In God we trust” has not been interpreted as violating the establishment clause and the Supreme Court has never ruled specifically on it. Might be unlikely though given that it does not specifically specify any religion but I am no legal scholar.
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