When bacteria are stained using a method called Gram staining, they can be classified into two groups: gram positive and gram negative. Bacteria that are gram positive will appear purple under a microscope, while bacteria that are gram negative will appear pink. The difference between these two groups has to do with the structure of the cell wall. Gram positive bacteria have a cell wall made up of a single layer of peptidoglycan, while gram negative bacteria have a cell wall made up of two layers of peptidoglycan. This difference in cell wall structure affects the way that the bacteria interact with the environment around them.
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