When animals use ecolocation, they send out a sound and listen for an echo. By comparing the sound they sent out to the echo that came back, they can learn information about the environment.
The loudness of the echo, the amount of time it takes to hear the echo, and the frequency spectrum of the echo (the pitches included) allow animals to estimate the distance to an object, and also get an idea of its size and shape.
So a bat, for instance, knows what a moth echo sounds like and can detect where the moth is based on that echo.
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