Biological sex is determined by a person’s genome, which is defined in the moment or conception, when gametes from each of their parents become a zygote (a full set of DNA). What’s in that DNA will determine how they develop.
For most people, it comes down to whether they got an X or Y chromosome from their father, but there are some exceptions outside XX and XY.
We’re not like crocodiles or whatever where our incubation temperature can change which sex we turn out to be. 😅
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