Its mostly old organic matter, oils, skin cells, etc, that has attached itself to the grout (the stuff between tiles). It will have been colonized by bacteria but doesnt mean that they are ‘bad’ bacteria. The best way to clean it spotless is with a pressure washer. Scrubbing can work but grout is rough like sandpaper, your brush will not get the ‘dark’ stuff our because it is mashed in there.
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