In C3 plants, the bundle sheath cells do not contain chloroplasts. In C4 plants, the bundle sheath cells contain chloroplasts. In C3 plants, the carbon dioxide fixation takes place only at one place. In C4 plants, the carbon dioxide fixation takes place twice
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There are 3 distinct different ways of doing photosynthesis C3, C4 and CAM and water loss is the driving force in the general difference along with access to carbon dioxide. https://youtu.be/xvj2i5F1U54
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